Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Foxo1

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Virus-peptide-specfic CD8 T cell (P14)
cell type
P14 T cells
time
d0
condition
naive
genotype
WT
chip antibody
with FOXO1 Ab

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
FOXO1 ChIP-seq was performed as before with minor modifications (Lin et al., 2010). Briefly, 3 x 107 T cells were fixed for 5 to 10 minutes at room temp in 1% formaldehyde then resuspended in lysis buffer. Following adaptor ligation, the DNAs were size selected (200-300bp) by 8% PAGE and index primers added by PCR. Samples were purified by 8% PAGE and precipitated with ethanol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
27584438
Reads aligned (%)
95.5
Duplicates removed (%)
54.9
Number of peaks
2514 (qval < 1E-05)

mm9

Number of total reads
27584438
Reads aligned (%)
95.3
Duplicates removed (%)
55.0
Number of peaks
2505 (qval < 1E-05)

Base call quality data from DBCLS SRA